Form: 10-K

Annual report pursuant to Section 13 and 15(d)

March 28, 2024

Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the Registration Statements on Form S-8 (File Nos. 333-168820, 333-190480, 333-197937, 333-206569, 333- 221473, 333-228253, 333-231520, 333-236059, 333-238505, 333-254156, 333-263421 and 333-270625), Form S-1 (File Nos. 333-228596, 333-232817, 333-234530, 333-238970, 333-250074 and 333-251088) and Form S-3 (File Nos. 333-261811, 333-269059 and 333-272641) of Achieve Life Sciences, Inc. of our report dated March 28, 2024 relating to the consolidated financial statements, which appears in this Form 10-K.

/s/PricewaterhouseCoopers LLP

Chartered Professional Accountants


Vancouver, Canada
March 28, 2024