Form: 10-K/A

Annual report pursuant to Section 13 and 15(d)

May 16, 2023

Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in the registration statements on Form S-8 (File Nos.333-153206, 333-168820, 333-190480, 333-197937, 333-206569, 333-221473, 333-228253, 333-231520, 333-236059, 333-238505, 333-254156 and 333-263421), Form S-1 (File Nos. 333-228596, 333-232817, 333-234530, 333-238970 and 333-250074) and Form S-3 (File Nos. 333-269059and 333-261811) of Achieve Life Sciences, Inc. of our report dated March 16, 2023 relating to the consolidated financial statements, which appears in the Annual Report on Form 10-K for the year ended December 31, 2022.

/s/ PricewaterhouseCoopers LLP

Chartered Professional Accountants

Vancouver, Canada
May 16, 2023